High-density mapping for gray leaf spot resistance using two related tropical maize recombinant inbred line populations

Gray leaf spot (GLS) caused by Cercospora zeae-maydis or zeina is one of the devastating maize foliar diseases worldwide. Identification GLS-resistant quantitative trait loci (QTL)/genes plays an urgent role in improving GLS resistance breeding practice. Two groups recombinant inbred line (RIL) populations derived from CML373 × Ye107 and Chang7-2 were generated subjected to genotyping-by-sequencing (GBS). A total 1,929,222,287 reads (RIL-YCML) 2,585,728,312 (RIL-YChang), with average 10,961,490 13,609,096 (RIL-YChang) per individual, got, which was roughly equal 0.70-fold 0.87-fold coverage B73 RefGen_V4 genome for each F7 respectively. 6418 5139 SNP markers extracted construct two high-density genetic maps. Comparative analysis using these physically mapped marker demonstrated a satisfactory colinear relationship reference genome. 11 QTL have been detected. The individual accounted 1.53–24.00% phenotypic variance explained (PVE). new consensus (qYCM-DS3-3/qYCM-LT3-1/qYCM-LT3-2) largest effect located chromosome bin 3.05, interval 2.7 Mb, representing 13.08 24.00% PVE. Further gene annotation indicated that there four candidate genes (GRMZM2G032384, GRMZM2G041415, GRMZM2G041544, GRMZM2G035992) qYCM-LT3-1, may be related resistance. Combining RIL GBS-based maps, larger delimited narrow genomic interval, will provide source programs.